LOCUS       40924_49427        4914 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Boolean integrase logic vector XOR gate BCD-RFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Courbet A, Endy D, Renard E, Molina F, Bonnet J.
  TITLE     Detection of pathological biomarkers in human clinical samples via 
            amplifying genetic switches and logic gates
  JOURNAL   Sci Transl Med 7 (289), 289RA83 (2015)
  PUBMED    26019219
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Courbet A, Molina F, Endy D, Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la 
            Valsiere, Montpellier, Herault 34184, France
REFERENCE   3  (bases 1 to 4914)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4914)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere, 
            Montpellier, Herault 34184, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..69
                     /label=P7
                     /note="P7"
                     /regulatory_class="promoter"
     misc_feature    76..128
                     /label=attB TP901-1
                     /note="attB TP901-1"
     misc_feature    129..178
                     /label=attB Bxb1
                     /note="attB Bxb1"
     terminator      193..264
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      280..307
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    320..372
                     /label=attP Bxb1
                     /note="attP Bxb1"
     misc_feature    373..422
                     /label=attP TP901-1
                     /note="attP TP901-1"
     misc_RNA        424..474
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     misc_feature    complement(497..523)
                     /label=RiboJ
                     /note="RiboJ"
     misc_feature    498..582
                     /label=BCD2
                     /note="BCD2"
     RBS             568..576
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             580..1275
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     terminator      complement(1301..1344)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1385..1412
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1419..1439
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1475..1506
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      1631..1725
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2217..2439
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2487..3434
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     CDS             3726..4583
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4597..4656
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      4716..4745
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      complement(4839..4868)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agaaaaaatt tatttgcttt cgcatctttt tgtacctata
       61 atgtgtggaa agcttatgcc aacacaatta acatctcaat caaggtaaat gctttttgct
      121 ttttttgctc ggccggcttg tcgacgacgg cggtctccgt cgtcaggatc atccgggcca
      181 tatgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      241 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      301 ttctgcgttt ataatgcatg ggtttgtacc gtacaccact gagaccgcgg tggttgacca
      361 gacaaaccac gaaaaggagt tttttagtta ccttaattga aataaacgaa ataaaaactc
      421 gcagctgtca ccggatgtgc tttccggtct gatgagtccg tgaggacgaa acagcctcta
      481 caaataattt tgtttaaggg cccaagttca cttaaaaagg agatcaacaa tgaaagcaat
      541 tttcgtactg aaacatctta atcatgctaa ggaggttttc taatgtcaga attaattaaa
      601 gaaaatatgc acatgaaatt atatatggaa ggtactgtca acaatcatca tttcaaatgc
      661 acatccgaag gtgaaggtaa accatatgaa ggcacacaaa caatgcgcat caaagcagtt
      721 gaaggtggac ccctgccctt tgcgtttgac attctcgcaa cgagctttat gtacgggtct
      781 aaaactttta tcaatcacac ccaaggcatt cctgactttt ttaaacagtc ctttcctgaa
      841 ggctttacct gggaacgtgt aacaacttat gaagatggcg gtgtacttac agcaactcaa
      901 gatacgagtt tacaagatgg ctgtctgatt tacaatgtta aaatccgtgg cgtaaatttc
      961 ccgagtaacg gacccgtaat gcaaaaaaaa actcttggtt gggaagcatc aacagaaacc
     1021 ttatatcctg cggacggtgg cttagaagga cgcgcagaca tggcactgaa attagttgga
     1081 ggcggtcatt taatctgcaa cctgaaaaca acctatcgtt ccaaaaaacc cgctaaaaac
     1141 cttaaaatgc ctggagtata ctatgttgat cgtcgcttag aacgtattaa agaagctgat
     1201 aaagaaacct acgttgaaca acatgaagta gccgtagccc gttattgtga ccttccgtcg
     1261 aaattaggac atcgttgata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1321 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1381 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagtagcgg ccgctgcagg
     1441 agtcactaag ggttagttag ttagattagc agaaagtcaa aagcctccga ccggaggctt
     1501 ttgactaaaa cttcccttgg ggttatcatt ggggctcact caaaggcggt aatcagataa
     1561 aaaaaatcct tagctttcgc taaggatgat ttctgctaga gctgtcagac caagtttacg
     1621 agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
     1681 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag cactagggac
     1741 agtaagacgg gtaagcctgt tgatgatacc gctgccttac tgggtgcatt agccagtctg
     1801 aatgacctgt cacgggataa tccgaagtgg tcagactgga aaatcagagg gcaggaactg
     1861 ctgaacagca aaaagtcaga tagcaccaca tagcagaccc gccataaaac gccctgagaa
     1921 gcccgtgacg ggcttttctt gtattatggg tagtttcctt gcatgaatcc ataaaaggcg
     1981 cctgtagtgc catttacccc cattcactgc cagagccgtg agcgcagcga actgaatgtc
     2041 acgaaaaaga cagcgactca ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt
     2101 tgcccgagct tgcgagggtg ctacttaagc ctttagggtt ttaaggtctg ttttgtagag
     2161 gagcaaacag cgtttgcgac atccttttgt aatactgcgg aactgactaa agtagtgagt
     2221 tatacacagg gctgggatct attcttttta tcttttttta ttctttcttt attctataaa
     2281 ttataaccac ttgaatataa acaaaaaaaa cacacaaagg tctagcggaa tttacagagg
     2341 gtctagcaga atttacaagt tttccagcaa aggtctagca gaatttacag atacccacaa
     2401 ctcaaaggaa aaggacatgt aattatcatt gactagccca tctcaattgg tatagtgatt
     2461 aaaatcacct agaccaattg agatgtatgt ctgaattagt tgttttcaaa gcaaatgaac
     2521 tagcgattag tcgctatgac ttaacggagc atgaaaccaa gctaatttta tgctgtgtgg
     2581 cactactcaa ccccacgatt gaaaacccta caaggaaaga acggacggta tcgttcactt
     2641 ataaccaata cgctcagatg atgaacatca gtagggaaaa tgcttatggt gtattagcta
     2701 aagcaaccag agagctgatg acgagaactg tggaaatcag gaatcctttg gttaaaggct
     2761 ttgagatttt ccagtggaca aactatgcca agttctcaag cgaaaaatta gaattagttt
     2821 ttagtgaaga gatattgcct tatcttttcc agttaaaaaa attcataaaa tataatctgg
     2881 aacatgttaa gtcttttgaa aacaaatact ctatgaggat ttatgagtgg ttattaaaag
     2941 aactaacaca aaagaaaact cacaaggcaa atatagagat tagccttgat gaatttaagt
     3001 tcatgttaat gcttgaaaat aactaccatg agtttaaaag gcttaaccaa tgggttttga
     3061 aaccaataag taaagattta aacacttaca gcaatatgaa attggtggtt gataagcgag
     3121 gccgcccgac tgatacgttg attttccaag ttgaactaga tagacaaatg gatctcgtaa
     3181 ccgaacttga gaacaaccag ataaaaatga atggtgacaa aataccaaca accattacat
     3241 cagattccta cctacgtaac ggactaagaa aaacactaca cgatgcttta actgcaaaaa
     3301 ttcagctcac cagttttgag gcaaaatttt tgagtgacat gcaaagtaag catgatctca
     3361 atggttcgtt ctcatggctc acgcaaaaac aacgaaccac actagagaac atactggcta
     3421 aatacggaag gatctgaggt tcttatggct cttgtatcta tcagtgaagc atcaagacta
     3481 acaaacaaaa gtagaacaac tgttcaccgt tagatatcaa agggaaaact gtccatatgc
     3541 acagatgaaa acggtgtaaa aaagatagat acatcagagc ttttacgagt ttttggtgca
     3601 tttaaagctg ttcaccatga acagatcgac aatgtaacta ctagagctaa atacattcaa
     3661 atatctatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3721 agaatatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgccgaa gatcagttgg
     3841 gtgcacgtgt gggttacatc gaactggacc tcaacagcgg taagattctt gagagttttc
     3901 gccccgaaga acgtttccca atgatgagca cttttaaagt tctgctctgt ggcgcggtat
     3961 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4021 acttggttga gtactcacca gtcacagaaa agcatcttac ggacggcatg acagtacgcg
     4081 aattatgcag cgctgccata accatgagtg ataacacggc ggccaactta cttctgacaa
     4141 cgatcggagg accgaaggag cttaccgctt ttttgcacaa catgggtgat catgtaactc
     4201 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4261 cgatgcctgt agctatggca acaacgttgc gcaaactctt aactggcgaa cttcttactc
     4321 tcgcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4381 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4441 ggtcccgcgg tattattgca gccctggggc cagatggtaa gccctcccgt atcgtagtta
     4501 tctacacgac ggggagccag gcaactatgg acgaacgtaa tcgccagatc gctgagatag
     4561 gtgcctccct gattaagcat tggtaataat actagctccg gcaaaaaaac gggcaaggtg
     4621 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgtttg ccacctgacg
     4681 tctaagaaaa ggaatattca gcaatttgcc cgtgccgaag aaaggcccac ccgtgaaggt
     4741 gagccagtga gttgattgct acgtaattag ttagttagcc cttagtgact ctgccacctg
     4801 acgtctaaga aaaggaatat tcagcaattt gcccgtgccg aagaaaggcc cacccgtgaa
     4861 ggtgagccag tgagttgatt gctacgtaat tagttagtta gcccttagtg actc
//
